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Am. J. Enol. Vitic. 50:2:199-203 (1999)
Copyright © 1999 by the American Society for Enology and Viticulture.
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Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Analysis of Grape Anthocyanins

Janyce A. Sugui 1, Karl V. Wood 2, Zhiying Yang 3, Connie C. Bonham 3, and Ralph L. Nicholson 1

1 Departments of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana USA 47906.
2 Department of Chemistry, Purdue University, West Lafayette, Indiana USA 47906.
3 Department of Biochemistry, Purdue University, West Lafayette, Indiana USA 47906.

kvw{at}purdue.edu

Matrix-assisted laser desorption ionization mass spectrometry (MALDI) was used as a tool for developing a rapid and efficient screening technique for the presence and identification of anthocyanins in grape skins. The anthocyanins of ten different grape varieties were extracted with a simple extraction procedure. Each of the five red grape varieties exhibited a distinct mass spectrum. The most abundant anthocyanins detected were the monoglucosides and the coumaroyl-monoglucosides of cyanidin, peonidin, delphinidin, petunidin, and malvidin.

Key words: Matrix-assisted laser desorption ionization (MALDI), mass spectrometry, anthocyanins, grapes, cyanidin, peonidin, delphinidin, petunidin, malvidin

Submitted on July 9, 1998




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Copyright © 1999 by the American Society for Enology and Viticulture.